Improvement of a polymerase chain reaction assay for the detection of Echinococcus multilocularis DNA in faecal samples of foxes
Identifieur interne : 001114 ( Main/Exploration ); précédent : 001113; suivant : 001115Improvement of a polymerase chain reaction assay for the detection of Echinococcus multilocularis DNA in faecal samples of foxes
Auteurs : Ph. Monnier [France] ; F. Cliquet [France] ; M. Aubert [France] ; S. Bretagne [France]Source :
- Veterinary Parasitology [ 0304-4017 ] ; 1996.
English descriptors
- KwdEn :
- Animals, Animals, Wild, DNA Primers, DNA, Helminth (analysis), Echinococcosis (diagnosis), Echinococcosis (veterinary), Echinococcus (isolation & purification), Feces (microbiology), Foxes (microbiology), Parasite Egg Count, Polymerase Chain Reaction (methods), Polymerase Chain Reaction (veterinary), Reproducibility of Results, Sensitivity and Specificity.
- MESH :
- chemical , analysis : DNA, Helminth.
- chemical : DNA Primers.
- diagnosis : Echinococcosis.
- isolation & purification : Echinococcus.
- methods : Polymerase Chain Reaction.
- microbiology : Feces, Foxes.
- veterinary : Echinococcosis, Polymerase Chain Reaction.
- Animals, Animals, Wild, Parasite Egg Count, Reproducibility of Results, Sensitivity and Specificity.
Abstract
A polymerase chain reaction (PCR) method was developed in order to permit a sensitive and specific identification of Echinococcus multilocularis DNA from fox faecal specimens. In an attempt to overcome problems related to the presence of endogenous PCR inhibitors in faecal extracts, we investigated a DNA extraction technique using an anion binding resin (Gene-Fizz). This simple and rapid procedure was applied to 61 faecal samples. Compared with the traditional microscopic examination, the sensitivity of PCR using Gene-Fizz was 82.3% and the specificity was 95.5%. No PCR signal was obtained for 20 Echinococcus granulosus isolates, showing that this method allowed discrimination between E. multilocularis and E. granulosus. Large-scale epidemiological surveys of fox excrements may be possible by using Gene-Fizz treatment prior to PCR amplification reactions.
Url:
DOI: 10.1016/S0304-4017(96)01039-4
Affiliations:
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Le document en format XML
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In an attempt to overcome problems related to the presence of endogenous PCR inhibitors in faecal extracts, we investigated a DNA extraction technique using an anion binding resin (Gene-Fizz). This simple and rapid procedure was applied to 61 faecal samples. Compared with the traditional microscopic examination, the sensitivity of PCR using Gene-Fizz was 82.3% and the specificity was 95.5%. No PCR signal was obtained for 20 Echinococcus granulosus isolates, showing that this method allowed discrimination between E. multilocularis and E. granulosus. Large-scale epidemiological surveys of fox excrements may be possible by using Gene-Fizz treatment prior to PCR amplification reactions.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li><li>Île-de-France</li></region><settlement><li>Créteil</li><li>Malzeville</li></settlement></list><tree><country name="France"><region name="Grand Est"><name sortKey="Monnier, Ph" sort="Monnier, Ph" uniqKey="Monnier P" first="Ph." last="Monnier">Ph. Monnier</name></region><name sortKey="Aubert, M" sort="Aubert, M" uniqKey="Aubert M" first="M." last="Aubert">M. Aubert</name><name sortKey="Bretagne, S" sort="Bretagne, S" uniqKey="Bretagne S" first="S." last="Bretagne">S. Bretagne</name><name sortKey="Cliquet, F" sort="Cliquet, F" uniqKey="Cliquet F" first="F." last="Cliquet">F. Cliquet</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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